In contrast, the pronounced reduction in chloroplasts in IDLs was because of disappearance of chloroplasts from most guard cells plus some decrease in mesophyll cells, but also by the death of cells in the spongy mesophyll (Figs 2l & 3g,i,j; FDA staining data not shown). 2002). 2000). This isolation gives a partially purified fraction of mitochondria which retain a very good coupling; however, a subtraction of thylakoid content needs to be done to calculate the respiratory rates. In DP leaves, the Fv/Fm decreased only slightly during the treatment and reached 0.7 ± 0.06 after 6 d in darkness, indicating that most of the PSII reaction centers remained functional in this treatment. Involvement of Abscisic Acid in PSII Photodamage and D1 Protein Turnover for Light-Induced Premature Senescence of Rice Flag Leaves. The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Nucleotide diversity measures indicate that the origin of glutinous rice is associated with reduced genetic variation characteristic of selection at the Waxy locus; comparison with an unlinked locus, RGRC2, confirms that this pattern is specific to Waxy. 1995; Blighet al. However, the fact that several mutations have occurred following the splice donor site mutation (Figure 2) suggests that the glutinous phenotype probably did not arise in the very recent past. Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of The intron 1 splice donor site mutation is shown in boldface type. In addition, active respiration could also supply carbon skeletons needed for efficient redistribution of nitrogen. However, in mitochondria from both DPs and IDLs, the RCR tended to decrease relative to normally illuminated leaves. Cycling conditions were 94° (2 min); then 30 cycles of 94° (30 sec), 55° (30 sec), 72° (2 min); and a final extension of 72° (5 min). However, the presence of both active mitochondria and mitochondria undergoing degradation in the same tissues indicates that different populations can exist. MON-877Ø1-2 (MON87701) - Resistance to lepidopteran pests of soybean including velvetbean caterpillar (Anticarsia gemmatalis) and soybean … Thus, the indica accessions examined here are genetically much more diverse than the japonica varieties. cells between control leaves (light) and dark‐treated plant (DP). However, when the results were expressed on a protein basis, oxygen consumption by leaf discs from IDLs was in the same range as leaf discs from light samples and even higher after 6 d of treatment. 1990), which reveal the central importance not only of genes of major effect, but also of secondary modifier loci in the evolution of domestication phenotypes. [Progress in regulation of rice Wx gene expression]. 2006) and also during programmed cell death (PCD) of mesophyll cells from cucumber (Cucumis sativus L.) (Selga, Selga & Pavila 2005). The sequenced portion of RGRC2 spans three introns and two small exons; most of this region is noncoding. The authors thank the International Rice Research Institute for providing rice accessions, Zhaohui Xue of the Duke University East Asia Library Collection for references on glutinous rice origins, and members of the Purugganan laboratory and Carolyn J. Ferguson for critical reading of the manuscript. The splice donor site mutation maps to a single point on the Wx haplotype tree (Figure 2). Arabidopsis thaliana spectrum of the rhodamine 123 was previously obtained with a 405 nm Seit über 16 Jahren sind wir der digitale Experte und First Mover, wenn es um die weibliche Zielgruppe geht. The conserved mobility of mitochondria during leaf senescence reflects differential regulation of the cytoskeletal components in water. None of the treatments resulted in changes in Chl a/b (Fig. 2004 May;108(7):1200-4. doi: 10.1007/s00122-003-1564-x. Clipboard, Search History, and several other advanced features are temporarily unavailable. PFD, photon flux density. Moreover, the death of some mesophyll cells (FDA staining, data not shown) contributed to this reduction in mitochondrial number. Glutinous rice contains a G to T mutation at the 5′ splice site of Wx intron 1 that leads to incomplete post-transcriptional processing of Wx pre-mRNA (Wanget al. —Nucleotide polymorphisms at the Wx locus. Regulation of photosynthesis and transcription factor expression by leaf shading and re-illumination in Arabidopsis thaliana leaves. Mitochondria were isolated, and both respiratory rates and respiratory control ratios (RCRs) were calculated according to the method described by Keech, Dizengremel & Gardeström (2005). Discrimination between green fluorescent Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions. 6b), leaf discs from IDLs showed a similar oxygen consumption to the light samples after 2 and 4 d, and even a slightly higher O2 consumption after 6 d, whereas a significant reduction of respiration was still observed in DPs. A variable simple sequence repeat (SSR) region composed of 8-20 CT dinucleotide repeats was the only molecular variation observed in the 5′ untranslated exon 1 (Figure 1). Transient shade and drought have divergent impacts on the temperature sensitivity of dark respiration in leaves of Geum urbanum. Light response curve for electron transport rate (ETR) with DP samples (b) and for IDL samples (c) after 2, 4 and 6 d. The control is an average of light after 0, 2, 4 and 6 d. Values are mean (±SD) of three replicates. Thank you for sharing this Genetics article. 1998; Isshikiet al. For each primer set, two 50-μl reactions were carried out per individual. o This pattern suggests that the mutation could have arisen within tropical japonicas and then subsequently spread northward as the temperate japonicas came to be distributed across Northeast Asia. Epub 2007 Oct 25. Nonglutinous Wx splice donor mutants: Besides glutinous accessions, 17 nonglutinous landraces also carry the splice donor site mutation (Table 1; Figure 2). What can be inferred about the geographical origin(s) of glutinous rice? This study demonstrates the utility of phylogeographic analysis of genes associated with domestication in studying crop origins. However, further investigations need to be done to precise the origins and the actors of this cytoplasmic rearrangement. This approach offers a promising means of investigating the origin and diversification of traits accompanying both crop domestication and evolution in wild species. But their where times that after siesta, my mom will cook us maruya in our merienda before playing outside during summer time. At the same time, the prevalence of the mutation in Northeast Asian nonglutinous accessions (Table 1) highlights the fact that this mutation alone does not guarantee expression of the glutinous phenotype. 2020 Feb;182(2):870-881. doi: 10.1104/pp.19.01062. Why is it so sticky? DNA sequences were visually aligned, and all polymorphisms were rechecked from chromatograms or by resequencing, with special attention to low-frequency polymorphisms.
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