Visualization of postoperative WT and SPARC-/- collagen matrix by histochemical staining and multiphoton microscopy. Parameters for collagen and matrix intensity that were significantly different between operated and unoperated are shown. -, no significant change from unoperated, normal conjunctiva. 122(18), 3209–3213 (2009). D. C. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. L. Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. So, H. H. Tang, C. S. Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and twophoton microscopy,” J. Biomed. Fig. Has this market change affected the MV Roadmap? K. A. Jansen, A. J. Licup, A. Sharma, R. Rens, F. C. MacKintosh, and G. H. Koenderink, “The role of network architecture in collagen mechanics,” Biophys. Relative amount of collagen compared to the total AROI. National Medical Research Council (NHIC-I2D-1503041, NMRC/CG/015/2013, NMRC/CSA-SI/0001/2015); SERI-Lee Foundation Pilot Grant (R1493/76/2017). In fact, we currently plan to add a Solaris-only release of UniVerse 11.3.2 and UniData 8.2.2 with native Python integration, one of our most exciting features introduced at 11.3.1 and 8.2.1 for Windows and Linux customers. T. Hayakawa, Y. Hashimoto, Y. Myokei, H. Aoyama, and Y. Izawa, “Changes in type of collagen during the development of human post-burn hypertrophic scars,” Clin. [CrossRef], 20. R. Lattouf, R. Younes, D. Lutomski, N. Naaman, G. Godeau, K. Senni, and S. Changotade, “Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues,” J. Histochem. On day 14, there was apparent recovery of the collagen network with more steadily birefringent collagen strands, but these had adopted an uncharacteristic wave-like pattern that rose up vertically towards the epithelium or vice versa. The existence of an apparent pattern suggests that some fibroblasts which were laying down intense collagen tracks were not migrating randomly, but rather appeared to have been drawn from the episclera towards the conjunctival epithelium or vice versa. Collagen fiber dimensions are known to be altered in scars. Right panel, the data for operated tissues in the left panels were replotted here to reveal changes in operated levels across the time points. [Crossref], S. K. Law, S. P. Modjtahedi, A. Mansury, and J. Caprioli, “Intermediate-term comparison of trabeculectomy with intraoperative mitomycin-C between Asian American and Caucasian glaucoma patients: a case-controlled comparison,” Eye 21(1), 71–78 (2007). F. J. Ávila, P. Artal, and J. M. Bueno, “Quantitative discrimination of healthy and diseased corneas with second harmonic generation microscopy,” Trans. K. Norose, J. I. Clark, N. A. Syed, A. Basu, E. Heber-Katz, E. H. Sage, and C. C. Howe, “SPARC deficiency leads to early-onset cataractogenesis,” Invest. Enter your email address to subscribe to this blog and receive notifications of new posts by email. In the mouse postoperative conjunctiva, we noticed a marginal (less than 10%) but significant increase in fiber orientation variation on day 14, which may reflect the distinct collagen pattern seen in both the histochemical images and multiphoton scans at this time point. [Crossref], B. D. Cumming, D. L. McElwain, and Z. Upton, “A mathematical model of wound healing and subsequent scarring,” J. R. Biol. [CrossRef], 32. 6(D)). J. 59, 80–89 (2017). 13(9), 1135–1149 (2013). In this study, progressive changes in postoperative conjunctival collagen structure and morphology in the SHG images were quantified using a morphology-based quantification algorithm [23,24]. Between 2004-2008, Pete has held positions in sales, recruiting, certified financial planning, systems architect and project manager for a “smart home” company, and starting an import and electronic integration business abroad in Costa Rica. (A) Left, multiphoton multimodal image of a sample mouse conjunctiva after experimental surgery: SHG signal in green color revealed the collagen fibers while TPE signal in red color revealed other tissue constituents; middle, separated SHG image; right, separated TPE image. Each symbol represents one eye from one mouse of a total of five mice measured at each time point (N=5). P. Puolakkainen, A. D. Bradshaw, T. R. Kyriakides, M. Reed, R. Brekken, T. Wight, P. Bornstein, B. Ratner, and E. H. Sage, “Compromised production of extracellular matrix in mice lacking secreted protein, acidic and rich in cysteine (SPARC) leads to a reduced foreign body reaction to implanted biomaterials,” Am. This way, you can still get the “one-stop-shop” service you have come to expect from Oracle SPARC/Solaris, but now with SPARC/Oracle Enterprise Linux. 10. In particular, by combining previously reported molecular data [15] with the current SHG measurements, it is now possible to stage conjunctival scar development in the mouse model of conjunctival scarring (Fig. The green SHG signals clearly showed collagen fibril distribution embedded within the postoperative conjunctival matrix containing other intrinsic cellular/ other structural fluorescence that were revealed as red TPE signals (Fig. Long before Oracle’s acquisition, the Sun SPARC chipset that powered its hardware sales faced dwindling adoption rates starting in the early 2000s. Surg. 18(8), 1772 (2017). D. C. Tai, N. Tan, S. Xu, C. H. Kang, S. M. Chia, C. L. Cheng, A. Wee, C. L. Wei, A. M. Raja, G. Xiao, S. Chang, J. C. Rajapakse, P. T. So, H. H. Tang, C. S. Chen, and H. Yu, “Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and twophoton microscopy,” J. Biomed. 10. Visual Sci. Reticulation of the fibrillar collagen network and collagen density are known independent determinants of tissue stiffness [40–42]. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, “Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues,” Biophys. So, with Moore’s law you could get the performance which will show up probably in 2 to 4 years. All experiments with animals were approved by the Institutional Animal Care and Use Committee (IACUC) and treated in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement on the Use of Animals in Ophthalmic and Vision Research. (A) collagen area ratio (CAR), (B) collagen fiber density (CFD), (C) collagen texture contrast (CTCon), (D) collagen fiber orientation variation, (E) collagen reticulation index (CRI), (F) mean collagen fiber thickness, (G) mean collagen fiber length, (H) mean collagen fiber straightness. Ophthalmol. [Crossref], T. J. Shaw and P. Martin, “Wound repair at a glance,” J. We are so excited to be introducing ConnectiQ and WebConnect to the Rocket Community. Biosci. [Crossref], S. Xu, Y. Wang, D. C. S. Tai, S. Wang, C. L. Cheng, Q. Peng, J. Yan, Y. Chen, J. Protoc. Solaris on SPARC continues to see releases, with advances. In other words, the enhanced reticulation observed in the early time points may not be indicative of bona fide collagen branching. [Crossref], P. Campagnola, “Second harmonic generation imaging microscopy: applications to diseases diagnostics,” Anal. 1. Hence, it appears that collagen orientation became significantly more scattered on day 14 post-surgery but this was temporary as it returned to normal by day 21. Want access to exclusive webinars and content? Your email address will not be published. Overall, deficiency in SPARC likely leads to a mature postoperative scar that features greater reductions in collagen density, fine structure, fiber thickness, length and straightness compared to WT scar. The most likely reason for the lack of increase in CARD on days 2 and 7 is the dramatic difference in CAs which were dramatically larger than normal in the two earlier time points, and so down-rendering the reticulation values. U. S. A. We hope to hear your comments below. 10(6), 751–760 (2017). Opt. The authors declare no conflicts of interest. 4(2), 199–201 (2004). C. R. Drifka, A. G. Loeffler, K. Mathewson, G. Mehta, A. Keikhosravi, Y. Liu, S. Lemancik, W. A. Ricke, S. M. Weber, W. J. Kao, and K. W. Eliceiri, “Comparison of picrosirius red staining with second harmonic generation imaging for the quantification of clinically relevant collagen fiber features in histopathology samples,” J. Histochem.

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